谱析（上海）生物科技有限公司优势供应：E.coli Topoisomerase IV
2019/10/11 15:43:58
E.coli Topoisomerase IV
Topo IV(来自大肠杆菌)是通过在大肠杆菌中过表达parC和parE亚基，并采用Peng和Marians(19991)的方法进行纯化而制备的。parC和parE亚基经SDS-PAGE纯化至>95%纯度。topo IV在稀释缓冲液中作为异四聚体复合物提供。建议重新引用酶，以避免重复的冻融循环。储存于-80℃。所有酶都提供5X浓缩的分析缓冲液和稀释缓冲液，也可单独使用。Topo IV (from Escherichia coli) is prepared by overexpressing the parC and parE subunits in E. coli and purifying them by methods adapted from Peng and Marians, 19991.
The parC and parE subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80ºC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
| Cat No. | Product | Size |
| T4001 | E. coli Topo IV |
100 U |
| T4002 | E. coli Topo IV |
500 U |
| T4003 | E. coli Topo IV |
1000 U |
| T4004 | E. coli Topo IV |
2000 U |
提供全套服务产品的高科技公司。目前能够面向全国的科学工作者提
供超 100 万种的各类化学标准品，杂质对照品，植物单体，化学试剂，
实验室设备，色谱耗材等科研用品。总部位于上海，物流遍布中国各
地及欧美等国家，为用户提供专业化，快捷化的一站式服务
