谱析（上海）生物科技有限公司提供——25-OH Vitamin D2/D3 in Plasma and Serum by HPLC 质谱诊断方案
 
一、说明
The determination of 25-OH vitamin D2/ D3 is used for the assessment of the vitamin D status.The RECIPE HPLC complete kit enables a quick and reliable quantification of both analytes from plasma and serum. The sample preparation consists of a simple precipitation step, a time-consuming solid phase extraction is not required.The subsequent HPLC analysis is performed with an injection interval of 10 min only. For system check, calibration and internal quality control a ClinTest@ Test Solution, a ClinCal@ Serum Calibrator (traceable to NIST standard SRM 972a) and ClinChek@ Serum Controls are available. 
 
二、产品优势
（1）Quick sample preparation(no solid phase extraction required)
（2）Injection interval: 10 min
（3）Traceable to NIST SRM 972a
 
三、测试数据
Linearity:                  3.0 - 500 μg/I (D2)
2.6 - 500 μg/I (D3) 
Recovery:                   99 - 104 %
LOD:                        1.1 μg/I (D2)
1.0 μg/I (D3)
LLOQ:                       3.0 μg/I (D2)
                            2.6 μg/I (D3)
Intraassay precision:      2.7 % (D2)
                            2.5 % (D3)
Interassay precision:       3.7 % (D2)
                            3.7 % (D3) 
 
四、高效液相色谱参数
Pump:                       isocratic pump, flow rate: 1.0 ml/min
Injection volume:           50 μI
Injection interval:         10 min
UV-Detector:                264 nm  
HPLC-Thermostat:           40°C
 
五、样品制备
 
 
 
六、订购信息
 

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