谱析（上海）生物科技有限公司提供——Vitamin B1, B2 in Whole Blood and B6 in Plasma / Whole Blood (Combikit) 质谱诊断方案
 
一、说明
The combi kit enables the combined measurement of the vitamins B1, B2, and B6. It consists of the chromatography kit (order no. 27000) and appropriate sample preparation kits (ordernos. 27001, 27002, 27006). The chromatography is performed with a single column for all B-vitamins in subsequent series of measurement. For switching the parameter (e.g. vitamin B1 --> vitamin B2) the parameter settings have to be changed (see HPLC parameters). By selecting the appropriate sample preparation kits, the customer is flexible to choose the parameters ccording to his needs.
 
二、产品优势
（1）High flexibility:
1 Chromatography kit
+ 3 sample preparation kits
 
三、测试数据
Test data are available on request.
 
四、高效液相色谱参数 维生素B1
Pump (Mobile Phase A + B):  flow rate 1 ml/min ratio A: B = 93: 7
Pump (Reagent N):           flow rate 0.1 ml/min
Injection volume:           50 μI
Injection interval:         6 min
HPLC-Thermostat:            35°C
Retention times:        
TPP                        approx. 2.5 min 
 
五、高效液相色谱参数 维生素B2
Pump (Mobile Phase A + B):  flow rate 1 ml/min ratio A: B = 80: 20
Injection volume:           50 μI
Injection interval:         10 min
HPLC-Thermostat:            40°C
Detector (exc. / em.):      450 nm / 530 nm  
Retention times:                         
FAD                         approx. 2.8 min
 
六、高效液相色谱参数 维生素B6
Pump (Mobile Phase A + B):  flow rate 1 ml/min   ratio A: B = 100: 0
Pump (Reagent N):           flow rate 0.2 ml/min 
Injection volume:           50 μI
Injection interval:         10 min
HPLC-Thermostat:            25°C
Detector (exc. / em.):      370 nm / 470 nm  
Retention times:                         
PLP                         approx. 5.2 min
 
七、样品制备
样品制备维生素B1见第58页（第二十四个），维生素B2见第60页（第二十五个），维生素B6见第62页（第二十六个）
 
八、色谱图
参见第59页(维生素B1，第二十四个)、61页(维生素B2，第二十五个)和63页(维生素B6，第二十六个)的色谱图
 
九、订购信息
The combi kit consists of the chromatography kit (order no. 27000) and the separate sample preparation kits (order nos. 27001, 27002 and 27006).
 
十、色谱法
 
 
十一、样品制备
 

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