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2019/10/14 11:27:48

结核分枝杆菌拓扑异构酶I是一种能够放松超螺旋DNAI型拓扑异构酶。它是一个潜在的抗菌药物靶点,可用于筛选潜在的新型抗菌化合物。该蛋白在大肠杆菌中过表达后经色谱纯化得到。它是用稀释缓冲液供应的。储存于-80℃,酶液中分别提供5倍浓度的缓冲液和1倍稀释缓冲液。1 U of topo I将在37℃下30分钟内放松0.5 g超螺旋pBR322 DNA
Mycobacterium tuberculosis topoisomerase I is a bacterial type I topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by chromatography.
It is supplied in Dilution Buffer. Store at -80 °C.
The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately. 1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37 °C.
优势供应产品:

CatNo. Product Product description
MTT01 M.tuberculosis Topo I
 
100U
MTT02 M.tuberculosis Topo I
 
500U

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