谱析(上海)生物科技有限公司优势供应:P. aeruginosa Gyrase
铜绿假单胞菌回转酶是一种可将负超螺旋引入DNA的II型拓扑异构酶。它是喹诺酮类和香豆素类药物的靶点,可用于筛选潜在的抗菌化合物。其制备方法是在大肠杆菌中过表达亚基,然后进行纯化。在稀释缓冲液中以A2B2络合物的形式提供。储存于-80℃。所有酶都提供5X浓缩的分析缓冲液和稀释缓冲液,也可单独使用。1 U的gyrase将在37℃条件下30分钟内超螺旋化0.5 g的pBR322 DNA。
Pseudomonas aeruginosa gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 µg relaxed pBR322 DNA in 30 minutes at 37 °C.
优势供应产品:
CatNo. | Product | Product description |
PAG1001 | P. aeruginosa Gyrase |
100 U |
PAG5002 | P. aeruginosa Gyrase |
500 U |